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Am J Physiol Gastrointest Liver Physiol 283: G473-G480, 2002. First published March 6, 2002; doi:10.1152/ajpgi.00421.2001
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Vol. 283, Issue 2, G473-G480, August 2002

Immunolocalization of electroneutral Na+-HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> cotransporters in human and rat salivary glands

V. Gresz1,3, T.-H. Kwon1, H. Vorum2, T. Zelles3, I. Kurtz4, M. C. Steward5, C. Aalkjaer1, and S. Nielsen1

1 The Water and Salt Research Center and 2 Department of Biochemistry, University of Aarhus, DK-8000 Aarhus C, Denmark; 3 Department of Oral Biology, Semmelweis University of Budapest, Budapest 1089, Hungary; 4 Division of Nephrology, University of California, Los Angeles, California 90095; and 5 School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom

Patterns of salivary HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> secretion vary widely among species and among individual glands. In particular, virtually nothing is known about the molecular identity of the HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> transporters involved in human salivary secretion. We have therefore examined the distribution of several known members of the Na+-HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> cotransporter (NBC) family in the parotid and submandibular glands. By use of a combination of RT-PCR and immunoblotting analyses, the electroneutral cotransporters NBC3 and NBCn1 mRNA and protein expression were detected in both human and rat tissues. Immunohistochemistry demonstrated that NBC3 was present at the apical membranes of acinar and duct cells in both human and rat parotid and submandibular glands. NBCn1 was strongly expressed at the basolateral membrane of striated duct cells but not in the acinar cells in the human salivary glands, whereas little or no NBCn1 labeling was observed in the rat salivary glands. The presence of NBCn1 at the basolateral membrane of human striated duct cells suggests that it may contribute to ductal HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> secretion. In contrast, the expression of NBC3 at the apical membranes of acinar and duct cells in both human and rat salivary glands indicates a possible role of this isoform in HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> salvage under resting conditions.

bicarbonate transport; intracellular pH regulation


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