AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 284: G96-G106, 2003. First published September 4, 2002; doi:10.1152/ajpgi.00160.2002
0193-1857/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/G96    most recent
00160.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Voland, P.
Right arrow Articles by Scott, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Voland, P.
Right arrow Articles by Scott, D.
Vol. 284, Issue 1, G96-G106, January 2003

Interactions among the seven Helicobacter pylori proteins encoded by the urease gene cluster

Petra Voland1, David L. Weeks2, Elizabeth A. Marcus2, Christian Prinz1, George Sachs2, and David Scott2

1 Department of Medicine II, Technical University, 81675 Munich, Germany; and 2 Department of Physiology, University of California Los Angeles and Veterans Affairs Greater Los Angeles Health System, Los Angeles, California 90073

Survival of Helicobacter pylori in acid depends on intrabacterial urease. This urease is a Ni2+-containing oligomeric heterodimer. Regulation of its activity and assembly is important for gastric habitation by this neutralophile. The gene complex encodes catalytic subunits (ureA/B), an acid-gated urea channel (ureI), and accessory assembly proteins (ureE-H). With the use of yeast two-hybrid analysis for determining protein-protein interactions, UreF as bait identified four interacting sequences encoding UreH, whereas UreG as bait detected five UreE sequences. These results were confirmed by coimmunoprecipitation and beta -galactosidase assays. Native PAGE immunoblotting of H. pylori inner membranes showed interaction of UreA/B with UreI, whereas UreI deletion mutants lacked this protein interaction. Deletion of ureE-H did not affect this interaction with UreI. Hence, the accessory proteins UreE/G and UreF/H form dimeric complexes and UreA/B form a membrane complex with UreI, perhaps enabling assembly of the urease apoenzyme at the membrane surface and immediate urea access to intrabacterial urease to allow rapid periplasmic neutralization.

yeast two-hybrid; urease assembly; protein interactions.


This article has been cited by other articles:


Home page
 MicrobiologyHome page
S. L. Benoit, A. L. Zbell, and R. J. Maier
Nickel enzyme maturation in Helicobacter hepaticus: roles of accessory proteins in hydrogenase and urease activities
Microbiology, November 1, 2007; 153(11): 3748 - 3756.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. L. Benoit, N. Mehta, M. V. Weinberg, C. Maier, and R. J. Maier
Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation
Microbiology, May 1, 2007; 153(5): 1474 - 1482.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. S. Davis, E. L. Flannery, and H. L. T. Mobley
Helicobacter pylori HP1512 Is a Nickel-Responsive NikR-Regulated Outer Membrane Protein
Infect. Immun., December 1, 2006; 74(12): 6811 - 6820.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
Y. Wen, J. Feng, D. R. Scott, E. A. Marcus, and G. Sachs
Involvement of the HP0165-HP0166 Two-Component System in Expression of Some Acidic-pH-Upregulated Genes of Helicobacter pylori.
J. Bacteriol., March 1, 2006; 188(5): 1750 - 1761.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. B. Mulrooney, S. K. Ward, and R. P. Hausinger
Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease
J. Bacteriol., May 15, 2005; 187(10): 3581 - 3585.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Benoit and R. J. Maier
Dependence of Helicobacter pylori Urease Activity on the Nickel-Sequestering Ability of the UreE Accessory Protein
J. Bacteriol., August 15, 2003; 185(16): 4787 - 4795.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online