AJP - GI Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 284: G1084-G1092, 2003. First published January 29, 2003; doi:10.1152/ajpgi.00383.2002
0193-1857/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/G1084    most recent
00383.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bisschops, R.
Right arrow Articles by Tack, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bisschops, R.
Right arrow Articles by Tack, J.
Vol. 284, Issue 6, G1084-G1092, June 2003

Electrical stimulation reveals complex neuronal input and activation patterns in single myenteric guinea pig ganglia

R. Bisschops1, P. Vanden Berghe1, E. Bellon2, J. Janssens1, and J. Tack1

1 Center for Gastroenterological Research and 2 Medical Image Computing (Radiology - ESAT/PSI), Katholieke Universiteit Leuven, 3000 Leuven, Belgium

The myenteric plexus plays a key role in the control of gastrointestinal motility. We used confocal calcium imaging to study responses to electrical train stimulation (ETS) of interganglionic fiber tracts in entire myenteric ganglia of the guinea pig small intestine. ETS induced calcium transients in a subset of neurons: 52.2% responded to oral ETS, 65.4% to aboral ETS, and 71.7% to simultaneous oral and aboral ETS. A total of 41.3% of the neurons displayed convergence of oral and aboral ETS-induced responses. Responses could be reversibly blocked with TTX (10-6 M), demonstrating involvement of neuronal conduction, and by removal of extracellular calcium. omega -Conotoxin (5 × 10-7 M) blocked the majority of responses and reduced the amplitude of residual responses by 45%, indicating the involvement of N-type calcium channels. Staining for calbindin and calretinin did not reveal different response patterns in these immunohistochemically identified neurons. We conclude that, at least for ETS close to a ganglion, confocal calcium imaging reveals complex oral and aboral input to individual myenteric neurons rather than a polarization in spread of activity.

small bowel motility; calcium imaging; confocal microscopy; neuronal networks; fluo 3; calretinin; calbindin


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. E. Wunderlich, B. J. Needleman, Z. Chen, J. G. Yu, Y. Wang, I. Grants, D. J. Mikami, W. S. Melvin, H. J. Cooke, and F. L. Christofi
Dual purinergic synaptic transmission in the human enteric nervous system
Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G554 - G566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. Bisschops, P. Vanden Berghe, G. Sarnelli, J. Janssens, and J. Tack
CRF-induced calcium signaling in guinea pig small intestine myenteric neurons involves CRF-1 receptors and activation of voltage-sensitive calcium channels
Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1252 - G1260.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.