HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/3/G593    most recent 00421.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Wang, X.-Y. Articles by Sanders, K. M. Search for Related Content PubMed PubMed Citation Articles by Wang, X.-Y. Articles by Sanders, K. M.

NEUROREGULATION AND MOTILITY

PKC- translocation in enteric neurons and interstitial cells of Cajal in response to muscarinic stimulation

¹Department of Physiology and Cell Biology and ²Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557

Submitted 26 September 2002 ; accepted in final form 15 April 2003

Interstitial cells of Cajal in the deep muscular plexus (ICC-DMP) of the small intestine express excitatory neurotransmitter receptors. We tested whether ICC-DMP are functionally innervated by cholinergic neurons in the murine intestine. Muscles were stimulated by intrinsic nerves and ACh and processed for immunohistochemistry to determine these effects on PKC- activation. Under control conditions, PKC--like immunoreactivy (PKC--LI) was only observed in myenteric neurons within the tunica muscularis. Electrical field stimulation or ACh caused translocation of neural PKC--LI from the cytosol to a peripheral compartment. After stimulation, PKC--LI was found in spindle-shaped cells in the DMP. These cells were identified as ICC-DMP by Kit-LI and vimentin-LI. PKC--LI in ICC-DMP and translocation of PKC-LI in neurons were blocked by tetrodotoxin or atropine, suggesting that these responses were due to activation of muscarinic receptors. Western blots also confirmed translocation of PKC--LI. In conclusion, PKC- translocation is linked to muscarinic receptor activation in ICC-DMP and a subpopulation of myenteric neurons. These studies demonstrate that ICC-DMP are functionally innervated by excitatory motoneurons.

muscarinic receptor; enteric nervous system; gastrointestinal motility

This article has been cited by other articles:

				A. Garcia-Pascual, M. Sancho, G. Costa, and D. Triguero Interstitial cells of Cajal in the urethra are cGMP-mediated targets of nitrergic neurotransmission Am J Physiol Renal Physiol, October 1, 2008; 295(4): F971 - F983. [Abstract] [Full Text] [PDF]

				H. Chen, T. Ordog, J. Chen, D. L. Young, M. R. Bardsley, D. Redelman, S. M. Ward, and K. M. Sanders Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine Physiol Genomics, November 14, 2007; 31(3): 492 - 509. [Abstract] [Full Text] [PDF]

				E. E. Daniel, A. E. Yazbi, M. Mannarino, G. Galante, G. Boddy, J. Livergant, and T. E. Oskouei Do gap junctions play a role in nerve transmissions as well as pacing in mouse intestine? Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G734 - G745. [Abstract] [Full Text] [PDF]

				D. P. Poole and J. B. Furness PKC {delta}-isoform translocation and enhancement of tonic contractions of gastrointestinal smooth muscle Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G887 - G898. [Abstract] [Full Text] [PDF]

				J. W. Wegener, V. Schulla, A. Koller, N. Klugbauer, R. Feil, and F. Hofmann Control of intestinal motility by the Cav1.2 L-type calcium channel in mice FASEB J, June 1, 2006; 20(8): 1260 - 1262. [Abstract] [Full Text] [PDF]

				S. M. Ward, G. J. McLaren, and K. M. Sanders Interstitial cells of Cajal in the deep muscular plexus mediate enteric motor neurotransmission in the mouse small intestine J. Physiol., May 15, 2006; 573(1): 147 - 159. [Abstract] [Full Text] [PDF]