| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
INFLAMMATION/IMMUNITY/MEDIATORS
Departments of 1Pharmacology and 2Internal Medicine, The University of Iowa, Iowa City, Iowa 52242
Submitted 12 June 2003 ; accepted in final form 1 October 2003
Voltage-dependent potassium currents are important contributors to neuron excitability and thus also to hypersensitivity after tissue insult. We hypothesized that gastric ulcers would alter K+ current properties in primary sensory neurons. The rat stomach was surgically exposed, and a retrograde tracer (1,1'-dioctadecyl-3,3,3,3'-tetramethylindocarbocyanine methanesulfonate) was injected into multiple sites in the stomach wall. Inflammation and ulcers were produced by 10 injections of 20% acetic acid (HAc) in the gastric wall. Saline (Sal) injections served as control. Nodose or T9–10 dorsal root ganglia (DRG) cells were harvested and cultured 7 days later to record whole cell K+ currents. Gastric sensory neurons expressed transient and sustained outward currents. Gastric inflammation significantly decreased the A-type K+ current density in DRG and nodose neurons (Sal vs. HAc-DRG: 82.9 ± 7.9 vs. 46.5 ± 6.1 pA/pF; nodose: 149.2 ± 10.9 vs. 71.4 ± 11.8 pA/pF), whereas the sustained current was not altered. In addition, there was a significant shift in the steady-state inactivation to more hyperpolarized potentials in nodose neurons (Sal vs. HAc: -76.3 ± 1.0 vs. -83.6 ± 2.2 mV) associated with an acceleration of inactivation kinetics. These data suggest that a reduction in K+ currents contributes, in part, to increased neuron excitability that may lead to development of dyspeptic symptoms.
stomach; visceral inflammation; hyperalgesia; dorsal root ganglia; nodose ganglia; voltage clamp; 4-aminopyridine; dendrotoxin; K+ currents
This article has been cited by other articles:
|
|
 |
|
  A. M. Harriott and M. S. Gold Electrophysiological Properties of Dural Afferents in the Absence and Presence of Inflammatory Mediators J Neurophysiol, June 1, 2009; 101(6): 3126 - 3134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hayashi, K. Takimoto, M. B. Chancellor, K. A. Erickson, V. L. Erickson, T. Kirimoto, K. Nakano, W. C. de Groat, and N. Yoshimura Bladder hyperactivity and increased excitability of bladder afferent neurons associated with reduced expression of Kv1.4 {alpha}-subunit in rats with cystitis Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2009; 296(5): R1661 - R1670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dang, K. Lamb, M. Cohen, K. Bielefeldt, and G. F. Gebhart Cyclophosphamide-Induced Bladder Inflammation Sensitizes and Enhances P2X Receptor Function in Rat Bladder Sensory Neurons J Neurophysiol, January 1, 2008; 99(1): 49 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nurgali, T. V. Nguyen, H. Matsuyama, M. Thacker, H. L. Robbins, and J. B. Furness Phenotypic changes of morphologically identified guinea-pig myenteric neurons following intestinal inflammation J. Physiol., September 1, 2007; 583(2): 593 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kayssi, S. Amadesi, F. Bautista, N. W. Bunnett, and S. Vanner Mechanisms of protease-activated receptor 2-evoked hyperexcitability of nociceptive neurons innervating the mouse colon J. Physiol., May 1, 2007; 580(3): 977 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-Y. Xu, J. H. Winston, M. Shenoy, H. Yin, and P. J. Pasricha Enhanced excitability and suppression of A-type K+ current of pancreas-specific afferent neurons in a rat model of chronic pancreatitis Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G424 - G431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Peeters, J. Aerssens, R. de Hoogt, A. Stanisz, H. W. Gohlmann, K. Hillsley, A. Meulemans, D. Grundy, R. H. Stead, and B. Coulie Molecular profiling of murine sensory neurons in the nodose and dorsal root ganglia labeled from the peritoneal cavity Physiol Genomics, February 23, 2006; 24(3): 252 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Patberg, M. N. Obreztchikova, S. F. Giardina, A. J. Symes, A. N. Plotnikov, J. Qu, P. Chandra, D. McKinnon, S. R. Liou, A. V. Rybin, et al. The cAMP response element binding protein modulates expression of the transient outward current: Implications for cardiac memory Cardiovasc Res, November 1, 2005; 68(2): 259 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dang, K. Bielfeldt, K. Lamb, and G. F. Gebhart Gastric Ulcers Evoke Hyperexcitability and Enhance P2X Receptor Function in Rat Gastric Sensory Neurons J Neurophysiol, June 1, 2005; 93(6): 3112 - 3119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sugiura, K. Dang, K. Lamb, K. Bielefeldt, and G. F. Gebhart Acid-Sensing Properties in Rat Gastric Sensory Neurons from Normal and Ulcerated Stomach J. Neurosci., March 9, 2005; 25(10): 2617 - 2627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Flake, D. B. Bonebreak, and M. S. Gold Estrogen and Inflammation Increase the Excitability of Rat Temporomandibular Joint Afferent Neurons J Neurophysiol, March 1, 2005; 93(3): 1585 - 1597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Kang, K. Lamb, G. F. Gebhart, and K. Bielefeldt Experimentally induced ulcers and gastric sensory-motor function in rats Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G284 - G291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Beyak, N. Ramji, K. M. Krol, M. D. Kawaja, and S. J. Vanner Two TTX-resistant Na+ currents in mouse colonic dorsal root ganglia neurons and their role in colitis-induced hyperexcitability Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G845 - G855. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
