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MUCOSAL BIOLOGY

Restitution of the bullfrog gastric mucosa is dependent on a DIDS-inhibitable pathway not related to ion transport

Department of Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215

Submitted 11 September 2002 ; accepted in final form 3 November 2003

This study was conducted to determine the contribution of ion transport to restitution after injury in the gastric mucosa. For this, intact sheets of stomach from the bullfrog, Rana catesbeiana, were mounted in Ussing chambers. Restitution was evaluated in the presence or absence of ion transport inhibitors amiloride, DIDS, and bumetanide to block Na⁺/H⁺ exchange, / exchange and / co-transport, and Na⁺-K⁺-2Cl^- cotransport, respectively. Ion substitution experiments with Na⁺-free, Cl^--free, and -free solutions were also performed. Injury to the mucosa was produced with 1 M NaCl, and restitution was evaluated by recovery of transepithelial resistance (TER), mannitol flux, and morphology. Amiloride, bumetanide, Cl^--free, or -free solutions did not affect restitution. In Na⁺-free solutions, recovery of TER and mannitol flux did not occur because surface cells did not attach to the underlying basement membrane. In contrast, all aspects of restitution were inhibited by DIDS, a compound that inhibits Na⁺-dependent transport. Because -free solutions did not inhibit restitution, it was concluded that DIDS must block a yet undefined pathway not involved in ion transport but essential for cell migration after injury and restitution in the gastric mucosa.

Rana catesbeiana; sodium bicarbonate cotransport; injury and repair

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