| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
NEUROREGULATION AND MOTILITY
Department of Human Physiology and Centre for Neuroscience, Flinders University, Adelaide, South Australia, 5001, Australia
Submitted 8 October 2003 ; accepted in final form 5 January 2004
A number of transient receptor potential (TRP) channels has recently been shown to mediate cutaneous thermosensitivity. Sensitivity to warm and cool stimuli has been demonstrated in both human and animal gastrointestinal tract; however, the molecular mechanisms that underlie this have not been determined. Vagal afferent neurons with cell bodies in the nodose ganglion are known to mediate nonnociceptive sensation from the upper gut. In this study, isolated cultured nodose ganglion from the mouse neurons showed changes in cytoplasmic-free Ca2+ concentrations over a range of temperatures, as well as to icilin (a TRPM8 and TRPN1 agonist) and capsaicin (a TRPV1 agonist). RT-PCR was used to show the presence of six temperature-sensitive TRP channel transcripts (TRPV1–4, TRPN1, and TRPM8) in whole nodose ganglia. In addition, RT-PCR of single nodose cell bodies, which had been retrogradely labeled from the upper gut, detected transcripts for TRPV1, TRPV2, TRPV4, TRPN1, and TRPM8 in a proportion of cells. Immunohistochemical labeling detected TRPV1 and TRPV2 proteins in nodose ganglia. The presence of TRP channel transcripts and proteins was also detected in cells within several regions of the gastrointestinal tract. Our results reveal that TRP channels are present in subsets of vagal afferent neurons that project to the stomach and may confer temperature sensitivity on these cells.
primary afferent neurons; nodose ganglion; stomach; retrograde labeling
This article has been cited by other articles:
|
|
 |
|
  D. Ni and L.-Y. Lee Lack of potentiating effect of increasing temperature on responses to chemical activators in vagal sensory neurons isolated from TRPV1-null mice Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L897 - L904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Fajardo, V. Meseguer, C. Belmonte, and F. Viana TRPA1 Channels Mediate Cold Temperature Sensing in Mammalian Vagal Sensory Neurons: Pharmacological and Genetic Evidence J. Neurosci., July 30, 2008; 28(31): 7863 - 7875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bielefeldt and B. M. Davis Differential effects of ASIC3 and TRPV1 deletion on gastroesophageal sensation in mice Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G130 - G138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Steiner, V. F. Turek, M. C. Almeida, J. J. Burmeister, D. L. Oliveira, J. L. Roberts, A. W. Bannon, M. H. Norman, J.-C. Louis, J. J. S. Treanor, et al. Nonthermal Activation of Transient Receptor Potential Vanilloid-1 Channels in Abdominal Viscera Tonically Inhibits Autonomic Cold-Defense Effectors J. Neurosci., July 11, 2007; 27(28): 7459 - 7468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Cheng, F. Yang, C. L. Takanishi, and J. Zheng Thermosensitive TRPV Channel Subunits Coassemble into Heteromeric Channels with Intermediate Conductance and Gating Properties J. Gen. Physiol., March 26, 2007; 129(3): 191 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Andersson, M. Nash, and S. Bevan Modulation of the Cold-Activated Channel TRPM8 by Lysophospholipids and Polyunsaturated Fatty Acids J. Neurosci., March 21, 2007; 27(12): 3347 - 3355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bielefeldt, F. Zhong, H. R. Koerber, and B. M. Davis Phenotypic characterization of gastric sensory neurons in mice Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G987 - G997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Taylor-Clark and B. J. Undem Transduction mechanisms in airway sensory nerves J Appl Physiol, September 1, 2006; 101(3): 950 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Simon Ion channel proteins in neuronal temperature transduction: from inferences to testable theories of deep-body thermosensitivity Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R515 - R517. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang and G. J Barritt TRPM8 in prostate cancer cells: a potential diagnostic and prognostic marker with a secretory function? Endocr. Relat. Cancer, March 1, 2006; 13(1): 27 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ruan, Q. Gu, Y. R. Kou, and L.-Y. Lee Hyperthermia increases sensitivity of pulmonary C-fibre afferents in rats J. Physiol., May 15, 2005; 565(1): 295 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Gu, R.-L. Lin, H.-Z. Hu, M. X. Zhu, and L.-Y. Lee 2-Aminoethoxydiphenyl borate stimulates pulmonary C neurons via the activation of TRPV channels Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L932 - L941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang and G. J. Barritt Evidence that TRPM8 Is an Androgen-Dependent Ca2+ Channel Required for the Survival of Prostate Cancer Cells Cancer Res., November 15, 2004; 64(22): 8365 - 8373. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
