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Am J Physiol Gastrointest Liver Physiol 287: G211-G219, 2004. First published February 19, 2004; doi:10.1152/ajpgi.00347.2003
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INFLAMMATION/IMMUNITY/MEDIATORS

Generation and characterization of immortalized rat pancreatic stellate cells

Gisela Sparmann,1 Christine Hohenadl,2 Jens Tornøe,3 Robert Jaster,1 Brit Fitzner,1 Dirk Koczan,4 Hans-Jürgen Thiesen,4 Änne Glass,5 David Winder,2 Stefan Liebe,1 and Jörg Emmrich1

1Department of Gastroenterology, University of Rostock, D-18057 Rostock, 2Institute of Virology, University of Veterinary Sciences, A-1210 Vienna, Austria, 3NsGene A/S, DK-2750 Ballerup, Denmark, 4Institute of Immunology and 5Institute of Medical Informatics and Biometry, University of Rostock, D-18057 Rostock, Germany

Submitted 11 August 2003 ; accepted in final form 3 February 2004

Pancreatic stellate cells (PSCs) are involved in, among other things, the pathogenesis of pancreatic fibrosis. Here, we present the generation of immortalized PSCs 7 and 14 days after isolation by retroviral gene transfer of the SV40 large T antigen encoding region. Propagated cell lines [large T immortalized cells (LTC)-7, LTC-14] retained characteristics of primary cells in terms of morphology, responsiveness to mediators regulating cellular functions such as proliferation, and expression profile of a number of investigated genes. Whereas LTC-14 kept the morphological features of the differentiation status of the primary cells they were made of, LTC-7 appeared similar to an earlier stage. Thus the established cell lines represent a versatile tool to investigate various aspects of PSC biology.

SV40 large T antigen immortalization; cytokine; microarray analysis


Address for reprint requests and other correspondence: G. Sparmann, Dept. of Gastroenterology, Univ. Hospital of Rostock, Ernst-Heydemann-Str. 6, D-18057 Rostock, Germany (E-mail: gisela.sparmann{at}med.uni-rostock.de).




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