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Am J Physiol Gastrointest Liver Physiol 287: G50-G57, 2004. First published March 11, 2004; doi:10.1152/ajpgi.00076.2003
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MUCOSAL BIOLOGY

Neutrophils augment recovery of porcine ischemia-injured ileal mucosa by an IL-1{beta}- and COX-2-dependent mechanism

Donnie E. Shifflett,1 Frank G. Bottone, Jr.,2 Karen M. Young,1 Adam J. Moeser,1 Samuel L. Jones,1 and Anthony T. Blikslager1

1Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh 27606 and 2Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Submitted 14 February 2003 ; accepted in final form 9 March 2004

Polymorphonuclear neutrophils (PMNs) play a critical role in intestinal mucosal injury and repair. To study effects of PMNs on acutely injured mucosa, we applied PMNs isolated from circulation or peritoneal fluid from animals with chemically induced peritonitis to ischemia-injured porcine ileal mucosa. In preliminary experiments, PMNs enhanced recovery of transepithelial electrical resistance (TER), and this action was inhibited by pretreatment with the nonselective cyclooxygenase (COX) inhibitor indomethacin. Because COX-2 is upregulated by inflammatory mediators such as IL-1{beta}, which is released by PMNs, we postulated that PMNs enhance recovery of ischemia-injured mucosa by a pathway involving IL-1{beta} and COX-2. Application of 5 x 106 PMNs to the serosal surface of ischemia-injured mucosa significantly enhanced recovery of TER (P < 0.05), an effect that was inhibited by the selective COX-2 inhibitor NS-398 (5 µM) and by an IL-1{beta} receptor antagonist (0.1 mg/ml). Addition of 10 ng/ml IL-1{beta} to the serosal surface of injured tissues caused a significant increase in TER (P < 0.05) that was inhibited by pretreatment with NS-398. Western blot analysis of mucosal homogenates revealed dramatic upregulation of COX-2 in response to IL-1{beta} or peritoneal PMNs, and the latter was inhibited by an IL-1{beta} receptor antagonist. Real-time PCR revealed that increased mRNA COX-2 expression preceded increased COX-2 protein expression in response to IL-1{beta}. We concluded that PMNs augment recovery of TER in ischemia-injured ileal mucosa via IL-1{beta}-dependent upregulation of COX-2.

intestine; prostaglandin; transepithelial electrical resistance; tight junction


Address for reprint requests and other correspondence: A. T. Blikslager, College of Veterinary Medicine, North Carolina State Univ., 4700 Hillsborough St., Raleigh, NC 27606 (E-mail: Anthony_Blikslager{at}ncsu.edu).






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