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This Article Full Text Full Text (PDF) All Versions of this Article: 289/2/G361    most recent 00369.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Stadnicki, A. Articles by Colman, R. W. Search for Related Content PubMed PubMed Citation Articles by Stadnicki, A. Articles by Colman, R. W.

INFLAMMATION/IMMUNITY/MEDIATORS

Immunolocalization and expression of kinin B₁R and B₂R receptors in human inflammatory bowel disease

Departments of ¹Internal Medicine, ²Histology and Embryology, and ³Molecular Biology, Biochemistry, and Biopharmacy, Medical University of Silesia, Katowice, Poland; and ⁴Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania

Submitted 13 August 2004 ; accepted in final form 22 March 2005

Bradykinin is a mediator of inflammation, responsible for pain, vasodilation, and capillary permeability. Bradykinin receptor 1 (B₁R) and bradykinin receptor 2 (B₂R) are G protein-coupled receptors that mediate kinin effects. The latter is constitutive and rapidly desensitized; the former is induced by inflammatory cytokines and resistant to densensitization. The distribution of bradykinin receptors in human intestinal tissue was studied in patients with inflammatory bowel disease (IBD), namely ulcerative colitis (UC) and Crohn's disease (CD). Both B₂R and B₁R proteins are expressed in the epithelial cells of normal and IBD intestines. B₁R protein is visualized in macrophages at the center of granulomas in CD. B₂R protein is normally present in the apexes of enterocytes in the basal area and intracellularly in inflammatory tissue. In contrast, B₁R protein is found in the basal area of enterocytes in normal intestine but in the apical portion of enterocytes in inflamed tissue. B₁R protein is significantly increased in both active UC and CD intestines compared with controls. In patients with active UC, B₁R mRNA is significantly higher than B₂R mRNA. However, in inactive UC patients, the B₁R and B₂R mRNA did not differ significantly. Thus bradykinin receptors in IBD may reflect intestinal inflammation. Increased B₁R gene and protein expression in active IBD provides a structural basis of the important role of bradykinin in chronic inflammation.

ulcerative colitis; Crohn's disease; bradykinin