HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 289/6/G1007    most recent 00085.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Kimura, Y. Articles by Buddington, R. K. Search for Related Content PubMed PubMed Citation Articles by Kimura, Y. Articles by Buddington, R. K.

MUCOSAL BIOLOGY

Lumenal adenosine and AMP rapidly increase glucose transport by intact small intestine

¹Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi; ²Department of Pathology, The University of Chicago, Chicago, Illinios; and ³Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, Mississippi

Submitted 23 February 2005 ; accepted in final form 12 July 2005

Adenosine modulates the intestinal functions of secretion, motility, and immunity, yet little is known about the regulation of nutrient absorption. Therefore, we measured the carrier-mediated uptake of tracer D-[¹⁴C]glucose (2 µM) by everted sleeves of the mouse intestine after a lumenal exposure to adenosine and a disodium salt of AMP. Rates of glucose uptake by intact tissues increased almost twofold after a 7-min exposure to 5 mM adenosine (a physiological dose). The response was slightly more pronounced for AMP and could be induced by forskolin. The response to adenosine was blocked by theophylline and the A₂ receptor antagonist 3,7-dimethyl-1-proparglyxanthine but not by the A₁ receptor antagonist 8-phenyltheophylline. Glucose uptake by control and AMP-stimulated tissues was inhibited by phloridzin, implying that sodium-dependent glucose transporter 1 (SGLT1) is the responsive transporter, but the involvement of glucose transporter 2 (GLUT2) cannot be excluded. Of clinical relevance, AMP accelerated the systemic availability of 3-O-methylglucose after an oral administration to mice. Our results indicate that adenosine causes a rapid increase in carrier-mediated glucose uptake that is of clinical relevance and acts via receptors linked to a signaling pathway that involves intracellular cAMP production.

sodium-dependent glucose transporter 1; adaptation; regulation; nongenomic

This article has been cited by other articles:

				T. P. Wong, E. S. Debnam, and P. S. Leung Involvement of an enterocyte renin angiotensin system in the local control of SGLT1-dependent glucose uptake across the rat small intestinal brush border membrane J. Physiol., October 15, 2007; 584(2): 613 - 623. [Abstract] [Full Text] [PDF]

				R. Ducroc, T. Voisin, A. El Firar, and M. Laburthe Orexins Control Intestinal Glucose Transport by Distinct Neuronal, Endocrine, and Direct Epithelial Pathways Diabetes, October 1, 2007; 56(10): 2494 - 2500. [Abstract] [Full Text] [PDF]