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Am J Physiol Gastrointest Liver Physiol 295: G895-G903, 2008. First published September 4, 2008; doi:10.1152/ajpgi.90378.2008
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LIVER AND BILIARY TRACT

The proteome of rodent mesenteric lymph

Anubhav Mittal,1 Martin Middleditch,2 Katya Ruggiero,2 Christina M. Buchanan,2,3 Mia Jullig,2,3 Benjamin Loveday,1 Garth J. S. Cooper,2,3 John. A. Windsor,1 and Anthony R. J. Phillips1,2,3

1Department of Surgery, Faculty of Medicine and Health Sciences, 2School of Biological Sciences, and 3Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand

Submitted 16 June 2008 ; accepted in final form 1 September 2008

Mesenteric lymph contributes to normal homeostasis and has an emerging role in the pathogenesis of multiple organ dysfunction syndrome. The aim of this study was to define the proteome of normal rodent mesenteric lymph in the fasted and fed states. Eight male Wistar rats fed a standard rodent diet were randomized to two groups. Group 1 (fasted, n = 4) were fasted for 24 h before anesthetized collection of mesenteric lymph. Group 2 (fed, n = 4) were allowed ad libitum access to food before lymph collection. Mesenteric lymph was subjected to proteomic analysis using iTRAQ and liquid chromatography-tandem mass spectrometry (LC-MS/MS). One hundred fifty proteins, including 26 hypothetical proteins, were identified in this study. All proteins were identified in lymph from both the fasted and fed states. The relative distribution profiles of protein functional classes in the mesenteric lymph differed significantly from that reported for plasma. The most abundant classes identified in lymph were protease inhibitors (16%) and proteins related to innate immunity (12%). In conclusion, this study provides the first detailed description of the normal mesenteric lymph proteome in the fed and fasted states using iTRAQ and LC-MS/MS.

rat; fed; fasted


Address for reprint requests and other correspondence: A. R. J. Phillips, Level 4, Thomas Bldg., School of Biological Sciences, Univ. of Auckland, Auckland 1023, New Zealand (e-mail: a.phillips{at}auckland.ac.nz)






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