Submitted on June 16, 2008
Revised on August 25, 2008
Accepted on September 1, 2008
The proteome of rodent mesenteric lymph
Anubhav Mittal1, Martin Middleditch2, Katya Ruggiero1, Christina M Buchanan1, Mia Jullig1, Benjamin Loveday1, Garth J.S. Cooper3, John Albert Windsor1, and Anthony R. J. Phillips1*
1 University of Auckland
2 Univeristy of Auckland
3 Auckland University
* To whom correspondence should be addressed. E-mail: a.phillips{at}auckland.ac.nz.
Mesenteric lymph contributes to normal homeostasis and has an emerging role in the pathogenesis of multiple organ dysfunction syndrome. The aim of this study was to define the proteome of normal rodent mesenteric lymph in the fasted and fed states. Eight male Wistar rats fed a standard rodent diet were randomised to 2 groups. Group 1 ('fasted', n = 4) were fasted for 24 hours prior to anaesthetised collection of mesenteric lymph. Group 2 ('fed', n = 4) were allowed ad libitum access to food prior to lymph collection. Mesenteric lymph was subjected to proteomic analysis using iTRAQTM and liquid chromatography-tandem mass spectrometry. 150 proteins, including 26 hypothetical proteins were identified in this study. All proteins were identified in lymph from both the fasted and fed states. The relative distribution profiles of protein functional classes in the mesenteric lymph differed significantly from that reported for plasma. The most abundant classes identified in lymph were protease inhibitors (16%) and proteins related to innate immunity (12%). In conclusion, this study provides the first detailed description of the normal mesenteric lymph proteome in the fed and fasted states using iTRAQTM and LC MS/MS.
