Hepatocellular carcinoma (HCC) is regarded as a major global health care issue, and chronic hepatitis B virus infection is considered to be involved in pathogenesis of HCC. In order to increase knowledge of HCC pathogenesis, as well as discover potential novel molecules for anti-cancer therapy, mass spectrometry (MS) and iTRAQ were employed. The differences between 9 HBV-related HCC and adjacent non-HCC tissue specimens were studied. In total, 222 proteins were analyzed for differential expression in the two types of samples. Among these proteins, several were further confirmed by immunohistochemical, immunoblotting and real-time RT-PCR analysis. RNA interference induced down-regulation of glucose-6-phosphate dehydrogenase (G6PD) and decreased HBV replication by 5-fold by the IFN pathway. Decreased G6PD expression resulted in decreased hepatoma cell migration and invasion in cell culture. In summary, the investigation provides new information on pathogenesis of HBV infection, and suggests G6PD as a novel anti-HCC target. G6PD suppression may contribute to treatment strategies for inhibiting tumor progression.
- Copyright © 2014, American Journal of Physiology- Gastrointestinal and Liver Physiology