Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, the acute lymphatic pump response in the mesentery to a postprandial lipid meal has gone unexplored. In this study, we used the rat mesenteric collecting vessel as an in vivo model to quantify the effect of lipoproteins on vessel function. Lipid load was continuously monitored using the intensity of a fluorescent fatty-acid analogue which we infused along with a fat emulsion through a duodenal cannula. The vessel contractility was simultaneously quantified. We demonstrated for the first time that collecting lymphatic vessels respond to an acute lipid load by reducing pump function. High lipid levels decreased contraction frequency and amplitude. We also showed a strong tonic response through a reduction in the end diastolic diameter. We further characterized the changes in flow rate and viscosity, and showed that both increase postprandially. In addition, shear mediated Ca2+ signaling in lymphatic endothelial cells differed when cultured with lipoproteins. Together these results show that the in vivo response could be both shear and lipid mediated, and provide the first evidence that high post-prandial lipid has an immediate negative effect on lymphatic function even in the acute setting.
- pump function
- lipid absorption
- Copyright © 2015, American Journal of Physiology- Gastrointestinal and Liver Physiology